Isolation and culturing of B. braunii

This green alga was isolated by serial dilution and grown on Chu-13 nutrient medium solidified by 1.5% agar-agar. The composition of the Chu-13 medium was CaNO₃ (0.300 g/L), MgSO₄.7H₂O (0.025 g/L), CaCl₂.2H₂O (0.027 g/L), K₂HPO₄ (0.010 g/L), ferric citrate (0.0035 g/L), citric acid (0.0035 g/L), Na₂CO₃ (0.02 g/L), Na₂SiO₃.5H₂O (0.044 g/L), and some micronutrients.¹⁵ Algal colonies appearing after 3 weeks of incubation were isolated and inoculated into liquid medium. For growth experiments algal species was grown for algal biomass in an incubator at 27±1 °C, 1.2±0.2 klux light intensity, and 16:8 h light:dark cycle in nutrient medium. After standardization of optimal culture conditions in Chu-13 medium the best results of growth of green alga were found.