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Controlled cortical impact model

NF Naama Toledano Furman
AG Assaf Gottlieb
KP Karthik S. Prabhakara
SB Supinder Bedi
HC Henry W. Caplan
KR Katherine A. Ruppert
AS Amit K. Srivastava
SO Scott D. Olson
CJ Charles S. Cox, Jr.
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To establish TBI model in the rats, unilateral brain injury was induced using Impact One Stereotaxic Impactor (Leica Microsystems, Buffalo Grove, IL) a controlled cortical impact (CCI) device. Rats were anesthetized with 4% isoflurane/O2. After mounting their head in a stereotactic frame, they received a single impact to the right parietal association cortex of 1.0 mm depth of deformation with an impact velocity of 5.0 m/sec and a dwell time of 200 ms to achieve moderate to severe injury. The injury procedure was complete after the incision was closed with staples.

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