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Tamoxifen administration
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Tamoxifen (Tx, Sigma-Aldrich) was dissolved in corn oil (Sigma-Aldrich) at 37 °C to a final concentration of 20 mg/ml. Tx was administered via intraperitoneal injection as a single dose at approximately 5 mg/kg body weight. Pregnant mice were injected one day after IUE for the short-term analysis, whereas the pups were injected one day after birth in the long-term experiments. The mice were analyzed at least 3 days after injection.
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