PARP enzyme assay

DK David King
XL Xiao Dun Li
GA Gilberto S. Almeida
CK Colin Kwok
PG Polly Gravells
DH Daniel Harrison
SB Saoirse Burke
AH Albert Hallsworth
YJ Yann Jamin
SG Sally George
SR Simon P. Robinson
CL Christopher J. Lord
EP Evon Poon
DY Daniel Yeomanson
LC Louis Chesler
HB Helen E. Bryant
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Endogenous PARP-1 enzyme activity in IMR-32, SHEP-1 and Sheptet21N cells with MYCN ON or OFF was determined using PARP/Apoptosis Colorimetric Assay Kit (Trevigen, MD, USA) following manufacturer’s instructions. In each case PARP activity was measured in 500 cells. The results are presented as Units PARP/500 cells in artificial units.

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