Endogenous PARP-1 enzyme activity in IMR-32, SHEP-1 and Sheptet21N cells with MYCN ON or OFF was determined using PARP/Apoptosis Colorimetric Assay Kit (Trevigen, MD, USA) following manufacturer’s instructions. In each case PARP activity was measured in 500 cells. The results are presented as Units PARP/500 cells in artificial units.
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