2.5. TTC staining

Twenty‐four hours after the induction of PIT ischemia, the brains were removed and sectioned coronally into 2‐mm‐thick slices using blade and a metallic brain matrix. Brain slices were immersed in 1% triphenyltetrazolium chloride (TTC) solution in normal saline at 37°C for 10 minutes and shook every 3 minutes. ³² Brain slices were photographed using a scanner, and the infarct volume, which was not stained with TTC, was measured by ImageJ.