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4.6. Wound Healing Assay

MC Maria Laura Centomo
EP Elisa Prola
AM Alessandra Merlini
EB Enrico Berrino
LN Lucia Napione
CM Cristina Marsero
IG Ilaria Gerardi
DS Dario Sangiolo
MA Massimo Aglietta
LD Lorenzo D’Ambrosio
YP Ymera Pignochino
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To evaluate EphA2 involvement in cell migration, confluent monolayers of silenced EphA2- cell lines were scratch-wounded with a 200 µL micropipette tip. Dislodged cells and debris were removed by washing cells with medium. Images of wound closure were taken immediately after scratching and at 24 and 48 h post-wounding using an Axio Vert.A1-Inverted Microscope (Carl Zeiss, Aalen, Germany), equipped with the True Chrome HD II camera (Tiesselab, Milano, Italy). Cell migration was measured at relative scratch gaps by ImageJ software, calculating the ratio of the scratch gap at the given point in time and the original gap at 0 h.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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