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4.10. Oil Red O (ORO) Staining of Atrial Tissue and Quantification

HL Hsiang-Chun Lee
HL Hsin-Ting Lin
LK Liang-Yin Ke
YH Yi-Lin Hsiao
CC Chih-Sheng Chu
WL Wen-Ter Lai
SS Shyi-Jang Shin
CC Chu-Huang Chen
SS Sheng-Hsiung Sheu
BW Bin-Nan Wu
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Lipid accumulation was assessed by ORO staining of 10 μm paraffin sections of atrial tissues fixed in phosphate-buffered 4% paraformaldehyde. Color images were acquired using a micropublisher 3.3 RTV camera, saved as TIFF files, and analyzed using Image J software. A 100-µm2 grid overlay was generated over each image, and the area fraction (%), defined as (points over ORO)/(points over image)/100, was determined. The values were derived from the average over the entire area of atrial tissue in multiple animals for each experimental group (n = 3 for each).

Copyright and License information:  ©2016 by the authors; licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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