Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

NMR and HRESIMS Analysis

AN Akihiro Ninomiya
SU Syun-ichi Urayama
RS Rei Suo
SI Shiro Itoi
SF Shin-ichi Fuji
HM Hiromitsu Moriyama
DH Daisuke Hagiwara
request Request a Protocol
ask Ask a question
Favorite

Nuclear magnetic resonance (NMR) spectra were recorded on a 500-MHz ECA500 NMR spectrometer (JEOL Ltd., Tokyo, Japan) at 293 K. The NMR chemical shifts 1H and 13C were referenced to the solvent peaks: δH 3.30 and δC 49.0 for CD3OD (Eurisotop, Saint-Aubin, France). High-resolution electrospray ionization mass spectrometry (HRESIMS) analysis was performed using UPLC-SYNAPT G2 HDMS (Waters, Milford, MA, United States).

Tenuazonic acid (compound 1): 1H and 13C NMR data – see Supplementary Table S1; HRESIMS m/z: [M + H]+; calculated for C10H16NO3 198.1130, result was 198.1127.

Copyright and License information:  ©2020 Ninomiya, Urayama, Suo, Itoi, Fuji, Moriyama and Hagiwara.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A