For all isolated E. coli, the microdilution broth method in accordance with the guidelines in Clinical and Laboratory Standards Institute (CLSI) document M07-A9 was performed to determine the MICs of DANO. Moreover, the results were interpreted according the breakpoint which was established in the previous study [5]. The antimicrobial susceptibilities of E. coli to ciprofloxacin, ofloxacin, levofloxacin and norfloxacin were determined by disk diffusion method described by European Committee on Antimicrobial Susceptibility Testing (EUCAST). The interpretation of the result was according to EUCAST criteria.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.