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The previous day of sacrifice, all mice were set in a separated plastic cage for stabilizing for 1 h after sensitization with 200 μl of 0.5% DNCB. Scratching behavior of each group was recorded for 20 min. The number of scratching was counted by acknowledging the scratches only the back side of the body. The scratching episode of each group was counted by a blind test. The number of scratching of each mouse was averaged from the counts of three researchers.
Copyright and License information: ©2020 Jo, Kim, Lee, Lee and Yang
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