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2.9. Measurement of Tissue Cytokines

YW Ya-Yu Wang
CW Chih-Cheng Wu
CC Chun-Jung Chen
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Rats (n = 6 per group) were anesthetized with isoflurane (2–4%) and then decapitated. The dissected brains were separated into contralateral and ipsilateral hemispheres for the isolation of cortical tissues. The obtained contralateral and ipsilateral cortical tissues and gastrocnemius tissues were subjected to the measurement of Tumor Necrosis Factor-α (TNF-α) protein content using ELISA kits (R&D Systems, Minneapolis, MN, USA).

Copyright and License information:  ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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