Pyocyanin production was examined as described previously with modifications [25]. Bacterial cultures containing different concentrations of glucose (0, 50, 100, 150 and 200 mg/mL) were grown for 17 h, and 1 mL samples were then centrifuged at 13,000 rpm for 5 min. The supernatant was collected and extracted with 600 μL chloroform, and then mixed by vortex (2 × 10 s). After centrifugation at 13,000 rpm for 5 min, the chloroform phase was transferred to a sterile tube and mixed with 0.5 mL of 0.2 M HCl followed by gentle shaking to transfer the pyocyanin to the aqueous phase. Pyocyanin was determined by measuring the absorbance of the aqueous phase at OD510.
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