2.8. Cell Proliferation Assay

The GIST 882, 882R, or 48 cells were seeded at a density of 20,000 cells/well, and the GIST-T1 or T1R cells were seeded at a density of 10,000 cells/well in 96-well plates. The cells were cultured with 3-bromopyruvate (3-BP), gossypol, antimycin A, oligomycin A, or dimethyl sulfoxide (DMSO)-only medium (Sigma-Aldrich, Darmstadt, Germany). The cell confluence in each well was monitored continuously every 6 h using an IncuCyte S3 Live Cell Analysis System (Sartorius, Gottingen, Germany) until at least 72 h.