Isolation of rat aortic smooth muscle cells

XW Xiaolin Wu
FB Fang Bian
HH He Hu
TZ Tongjian Zhu
CL Chenyu Li
QZ Qing Zhou
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The rats were killed, then opened chest and chest skin. The aorta vessels of rats were removed and placed in a pre cooled DMEM medium. The vascular medium membrane was separated under a microscope. The separated middle membrane was cut and broken by surgical scissors and cultured in DMEN with 20% fetal bovine serum.

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