2.4. Intraocular pressure measurement

Briefly, Mice were gently restrained by first being placed in a soft, clear plastic cone (Decapicone; Braintree Scientific, Inc., Braintree, MA), then secured in a custom-made restrainer. The restrained animal was then placed on a platform of adjustable height. After the mice were acclimated, IOP measurements were performed using the rebound tonometer (Colonial Medical Supply, Franconia, NH). All IOP measurements were performed during the light-on phase. IOP was measured for mutants as well as their age matched wildtype littermates at approximately the same time of day.