2.13. Paw Edema Measurement

In all experimental models, paw edema was analyzed by plethysmometry. The animals had the initial volume (Vi) of their right and left hind paws evaluated before the treatments. Then, differences between the final volume and the basal volume of the paws at each time was calculated using the formula: Ve.p.d/e = Vf − Vb, in which Ve.p.d/e = right/left paw edema volume (in μL), Vf = final volume of the right and left paw/time, and Vb = basal volume of the right and left paw/time. Afterwards, the Δ (delta) of the following formula was calculated: Δ = Ve.p.d − Ve.p.e. The percentage of inflammation between treated and control groups was calculated using the following formula: Inflammation (%): 100 × Δmt/Δmc, in which Δ mc and Δ mt represent the mean paw volume in the control and treated groups, respectively. Percent inhibition was calculated as: 100%—percentage of inflammation.