Observation of stained cells by bright field microscope

KY Kyohei Yamashita
ET Eiji Tokunaga
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The bright field microscopes and objective lenses used in each experiment are as follows6 (Tables (Tables7,7, ,88).

Measurement of absorbance of single living or dead cells (Figs. 1, ,22)

Inverted research microscope (IX71, OLYMPUS) with the 40 × or 100 × objective lens.

Other experiments.

Digital biological microscope (GR-D8T2, Shodensha, Inc.) with the 4 × or 10 × objective lens.

Conditions for microscopic observation (cell observation and absorption spectral imaging).

※ Magnification of the objective lens: Details of the objective lenses are shown in the following table (Table (Table88).

ASI Absorbance spectral imaging, MW microwave treated dead cells, BC Benzalkonium chloride treated dead cells.

Specifications of objective lenses.

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