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Luciferase Reporter Assays

YM Yang Ma
DC Dongqian Cui
YZ Yu Zhang
CH Chen-chen Han
WW Wei Wei
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Briefly, an appropriate amount of the EGR1 promoter-luciferase reporters, together with renilla luciferase plasmids, which served as the internal control, were co-transfected into HepG2 or HCCLM3 cells. 12 hrs later, cells were pretreated with 2 mg/mL integrin β1-neutralising antibody for 0.5 h and then with 250 ng/mL IGFBP2 for the following 12 h, then the cell lysates were prepared and subjected to Dual-luciferase Reporter Assay (Promega) according to the manufacturer’s instructions. Luciferase activities were normalized to renilla luciferase activities. Data represent at least three independent experiments.

Copyright and License information:  ©2020 Ma et al.
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