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CD34+ cell sorting

XZ Xiaojia Zhou
SL Simin Liang
QZ Qian Zhan
LY Li Yang
JC Jianxiang Chi
LW Li Wang
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Normal bone marrow CD34+ cells and AML bone marrow CD34+ cells were isolated from BMMCs by using EasySepTM Human CD34 Positive Selection Kit II (Stemcell Technologies, Canada). Briefly, 1 × 108 cells were incubated with 20 μL Selection Cocktail for 30 min at room temperature. Then, the cells were incubated in a magnet with 15 μL RapidSphereTM for 5 min at room temperature. After pouring off the supernatant, cells were resuspended in 1 mL of PBS to pick up the magnet coupled with CD34+ cells in five times in order to yield a high purity (>95%). Finally, the purity of collected CD34+ cells was estimated by flow cytometry (FACSCalibur analyzer, BD Biosciences, USA).

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