Murine Systemic Candidiasis Model and Treatment

C. albicans SC5314 was cultured in YPD medium at 30°C for 12 h. After washing them three times with PBS, cells were resuspended in physiological saline. Infection was induced by injecting a C. albicans suspension into the lateral tail vein (5 × 10⁵ CFU/mouse). 11g (4 mg/kg) or vehicle was administered intraperitoneally 2 h later and was injected once a day for 5 consecutive days (Hata et al., 2011). Survival rates were monitored for 35 days following infection. For measuring fungal burden, serial dilutions of paired kidneys and livers were plated on SDA plates on Day 5 after infection, and further incubated at 30°C for 48 h. For histopathological analysis, paired kidneys were fixed in 10% neutral buffered formalin on Day 5 after infection, and then stained with hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS) to reveal inflammatory cell infiltration and fungal hyphae. Three independent experiments were performed.