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FITC-Annexin V Apoptosis kit was used. U87 MG cells received the above treatments for 24 h. Trypsin was added to digest U87 MG cells, and then the cells were harvested. Next, cells were rinsed with PBS and re-suspended in binding buffer. After the cells were incubated in the presence of FITC-Annexin V and PI for 15 min, the number of apoptotic cells was calculated using a flow cytometer (BD, CA, USA).
Copyright and License information: ©2020 Zhao et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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