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Relative estimate of intracellular calcium was analyzed using Fura2-Am Reagent (Molecular Probes). Briefly, the cells were treated with drugs for 6 hours followed by incubation with Fura2-Am reagent (1 μmol/L) for 1 hour. The cells were washed and incubated further for 30 minutes. The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices).
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