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KT Kaylyn D. Tousignant
AR Anja Rockstroh
BP Berwyck L. J. Poad
AT Ali Talebi
RY Reuben S. E. Young
AF Atefeh Taherian Fard
RG Rajesh Gupta
TZ Tuo Zang
CW Chenwei Wang
ML Melanie L. Lehman
JS Johan V. Swinnen
SB Stephen J. Blanksby
CN Colleen C. Nelson
MS Martin C. Sadowski
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LNCaP (ATCC, CVCL_0395) and C4-2B (ATCC, CVCL_4784) cells were cultured in RPMI medium (Thermo Fisher) supplemented with 5% FBS. Medium was changed every 3 days and cells were incubated at 37 °C in 5% CO2. For treatments representing androgen-deprivation therapy, cells were cultured in RPMI medium supplemented with 5% charcoal -stripped serum (CSS). For AR-targeted therapies, cells were cultured in 5% or 10% FBS with AR antagonist enzalutamide (Enz, 10 μM). Cells were passaged at approximately 80% confluency by trypsinization. Cell lines were genotyped in March 2018 by Genomics Research Centre (Brisbane) and routinely tested for mycoplasma infection.

Copyright and License information: The Author(s) ©2020
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