4.3. ATP Measurement Assay

1.2 × 10⁴ (MDA-MB-231) or 2 × 10⁴ (MCF-7) cells were seeded in 96-well plates. Cells were stimulated in a volume of 50 µL. Measurements were performed according to the manufacturer’s instructions (CellTiter-Glo^® Luminescent Cell Viability Assay, Promega, Germany) with the addition of 50 µL CellTiter-Glo^® solution to each well. The luminescence intensity was analyzed in duplicate using the microplate reader Infinite M200pro (Tecan, Männedorf, Switzerland). The values were normalized against the viability of untreated cells, which was set as one relative unit (RU).