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Construction of hsa-miR-3938 and hsa-miR-4780 Lentiviral Vector
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CRCs were transfected with miR-4780 mimic, miR-4780 mimic NC, miR-3938 inhibitor, miR-3938 inhibitor NC using siRNA and siRNA-MateTM transfection reagent (ribo FECT ™ CP Transfection Kit). Briefly, 50–100 nM siRNA and 5ug/mL polybrene were mixed together and then added to cells that were 50–60% confluent. At 24–36 h after transfection, cells were collected for subsequent experiments.
Copyright and License information: ©2020 Wang et al.
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