2.1. Harvest of Basidiomata

The seven P. eryngii strains used in this study (Table 1) were grown on a sterile substrate (a mixture of corncob, grass, and sawdust, 65% moisture) according to Sánchez and Royse [16]. Basidiomata were produced at El Colegio de la Frontera Sur (ECOSUR), located in Tapachula, Chiapas, Mexico. Fruiting bodies were harvested when the pileus reached maximum extension, were cut longitudinally, and were subjected to natural drying in the shade.

Challenge of H. contortus larvae L3 with P. eryngii.

n = 5. ¹Methanol (negative control); ²IVM (positive control).