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PCR primer of all LKB1 exons and reaction set up was according to previous published literature [23]. The PCR product was loaded to 2% agarose gel and purified by TIANgel Mini Purification Kit (TIAGEN), and then sent for Sanger sequencing (Sangon Biotech). The result was aligned with reference genomic sequence of LKB1 (GRCh37.p13) and all SNPs were excluded through crosscheck with NCBI SNP database.

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