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4.3. UVA Irradiation of HaCaT Keratinocytes and HDFs

CK Chang-Suk Kong
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HaCaT keratinocytes and HDFs were irradiated by UVA using a Bio-Sun UV Irradiation System (Vilber Lourmat, Marine, France) fitted with a UVA source designed for microplates. Cells grown in microplates were irradiated at a 10 J/cm2 UVA dose. Cells were irradiated in phosphate-buffered saline (PBS) without the plastic lid. When the irradiation received matched the desired programmed energy, the UVA irradiation stopped automatically, and, subsequently, the cells were incubated with their own growth medium without FBS until analysis.

Copyright and License information:  ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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