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Cell viability assay

JR Julian Roewe
GS Georgios Stavrides
MS Marcel Strueve
AS Arjun Sharma
FM Federico Marini
AM Amrit Mann
SS Stephanie A. Smith
ZK Ziya Kaya
BS Birgit Strobl
MM Mathias Mueller
CR Christoph Reinhardt
JM James H. Morrissey
MB Markus Bosmann
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Macrophages were cultured in RPMI 1640 medium without phenol red (Thermo Fisher Scientific) supplemented with 100 U/ml penicillin-streptomycin and 0.1% (w/v) BSA with S-/L-polyphosphates (50 µM) for 24 h. To evaluate cell viability, the lactate dehydrogenase (LDH) activity in supernatants was measured using a colorimetric CytoTox 96® assay (Promega) according to the manufacturer’s instructions. As positive controls, untreated resting macrophages were disrupted in lysis buffer and used along with samples of supernatants. After 30 min, the substrate reaction was stopped and formazan generation measured at a wavelength of 490 nm (Opsys MR Microplate Reader).

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