3.10. Immunofluorescence Staining

Cells were fixed by 4% (v/v) paraformaldehyde and permeabilized by 0.1% (v/v) Triton X-100. Cells were incubated in blocking buffer containing 1% bovine serum albumin. After blocking, cells were incubated overnight with indicated primary antibodies against TOM20 (a mitochondria marker) and then incubated with Alexa Fluor-conjugated secondary antibodies. The nucleus was stained with DAPI, actin was stained with phalloidin, and cells were mounted using Prolong Gold reagent. Images were taken by LSM800 confocal microscopes (Zeiss).