Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET) Assays and IC50 Measurements
Ask a
question
Favorite
The binding affinities and IC50 values of the tested BET family member inhibitors for the BRD4 and BRD2 proteins bromodomains (BDs) were detected using a commercially available TR-FRET assay kits (Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer’s instructions and our recent publications (Borysko et al., 2018; Liu et al., 2018; Liu et al., 2020). The specificity of the designed and synthesized compounds for BRD4 was also evaluated through comparison with those for BRD2.
Copyright and License information: ©2020 Zhou, Li, Liu, Huang, Yao, Li, Chen, Zhang, Zhou, Wang and Zhang
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
