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Mice were fasted for 2 hours. Serum was collected before the FITC-dextran administration as the blank. Then, the mice were gavage-fed FITC-dextran (44 mg/100 g, FD4; Sigma, St. Louis, MO). Serum was collected again 1 hour afterward in conventional mice and 4 hours afterward in GF mice. Fluorescence was measured on samples diluted with PBS (1:2) using an excitation wavelength of 490 nm and an emission wavelength of 520 nm. Fold changes of FITC-dextran in the serum 1 hour/blank or 4 hours/blank were calculated.
Copyright and License information: ©2020 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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