2.6. Wire‐hanging test

The wire‐hanging test apparatus is comprised of a 50‐cm‐long 2‐mm diameter stainless‐steel bar placed on two vertical supports 37 cm above a flat surface. Mice were placed on the bar in the middle between two supports and were observed for 30 s with a total of four repeats. The amount of time spent hanging was recorded, and each mice was scored 0–4 according to its performance: 0, the mouse fell off the bar; 1, the mouse used 2 forepaws to hang on the bar; 2, the mouse hung onto the bar while also attempted to climb onto the bar; 3, the mouse hung onto the bar using not only 2 forepaws but also 1 or both hind paws; 4, the mouse hung onto the bar with all 4 paws and tail wrapped around the bar; and 5, the mouse was able to escape to either of the supports.