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Briefly, cells (4,000 cells/well) were seeded in a 96-well plate. Following culture for 48 h, the medium was removed and 10 µl MTT solution mixed with 110 µl fresh medium were added to each well. Following incubation at 37°C for 4 h, the medium was removed, and 150 µl DMSO was added to each well. The absorbance value at 570 nm was then detected using a microplate reader (PLUS 384, Molecular Devices, LLC).
Copyright and License information: ©2020 Wang et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
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