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Annexin V Apoptosis Assay

KM Kija Malale
JF Jili Fu
LQ Liewang Qiu
KZ Ke Zhan
XG Xiuni Gan
ZM Zhechuan Mei
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The differences in apoptosis were examined using an Annexin V apoptosis assay. Briefly, Huh7 and HepG2 cells were seeded in 6-well plates at 2 × 105 per well for 48 h under normoxic or hypoxic conditions prior to treatment. After sorafenib treatment/transfection, cells were harvested via trypsinization, washed twice with Phosphate buffered saline (PBS), and stained with a fluorescein isothiocyanate Annexin V Apoptosis Detection kit (BD Pharmingen, Franklin Lakes, NJ, USA). Flow cytometry (Thermo Fisher Scientific, Waltham, Massachusetts, USA) was used to determine the cell apoptosis ratio.

Copyright and License information:  ©2020 Malale et al.
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