TNF-α ELISA on Human Blood

Whole blood was supplied by the Scottish National Blood Transfusion Service (SNBTS), Glasgow, UK, and was preincubated for 48 h with varying concentrations of Q-actin or idoBR1, after which lipopolysaccharide (LPS) (10 μg/mL) was added, and incubations continued for a further 20 h. Following incubation at 37 °C (5% CO₂, 100% humidity), plasma was collected from the blood by centrifugation (30 s 10,000 g), and the levels of tumor necrosis factor alpha (TNF-α) in plasma samples were measured by enzyme-linked immunosorbent assay (ELISA). Values represent the mean ± sd of n = 3.