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Western blotting

JW Jing Wu
YY Yan-Qiu You
YM Yan-Xiu Ma
YK Yan-Hua Kang
TW Tian Wu
XW Xiang-Ji Wu
XH Xiao-Xiao Hu
QM Qiao-Hong Meng
YH Yin Huang
NZ Na Zhang
XP Xiao-Ben Pan
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Protein lysates (50 μg/lane) were resolved on appropriate SDS-PAGE gels and transferred to PVDF membrane (Millipore, Billerica, MA, USA). The membranes were blocked, incubated with the indicated primary antibodies at 4 °C overnight, and then with the appropriate horseradish peroxidase-conjugated secondary antibodies. Blots were quantified with Image J (National Institutes of Health, Bethesda, MD, USA). Primary antibodies from Abcam (Cambridge, USA) were used: DDX5 (#ab126730), cyclin A2 (#ab181591), CDK4 (#ab108357), cyclin D1 (#ab16663), PCNA (#ab92552). β-actin (#AC026, ABclonal, Wuhan, China) was used as an internal control.

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