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Measurement of Intracellular ROS
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Generation of intracellular ROS was measured by flow cytometer using dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescent probe, which can be oxidized by cellular oxidants into the highly fluorescent compound DCF. Therefore, the fluorescence intensity is equivalent to the level of peroxide generated by the cells. Cells were treated by JB for 24h, collected and washed. Then, they were re-suspended in RPMI 1640 and incubated with DCFH-DA for 30 min at 37°C in the dark. The fluorescence intensity was detected by flow cytometry.
Copyright and License information: ©2020 Pan et al.
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