Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Immunohistochemistry (IHC)

ZN Zeyad D Nassar
CM Chui Yan Mah
JD Jonas Dehairs
IB Ingrid JG Burvenich
SI Swati Irani
MC Margaret M Centenera
MH Madison Helm
RS Raj K Shrestha
MM Max Moldovan
AD Anthony S Don
JH Jeff Holst
AS Andrew M Scott
LH Lisa G Horvath
DL David J Lynn
LS Luke A Selth
AH Andrew J Hoy
JS Johannes V Swinnen
LB Lisa M Butler
request Request a Protocol
ask Ask a question
Favorite

Paraffin-embedded tissue sections (2–4 µm) were deparaffinized in xylene, rehydrated through graded ethanol, and blocked for endogenous peroxidase before being subjected to heat-induced epitope retrieval (Armstrong et al., 2016). IHC staining was performed using DECR1 (HPA023238, Sigma Aldrich, diluted 1:500) antibody and the 3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride (Sigma Aldrich) as described previously (Armstrong et al., 2016). Intensity of DECR1 immunostaining was measured by video image analysis (Armstrong et al., 2016).

Copyright and License information:  ©2020, Nassar et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A