For the reporter assay, cardiomyocytes were cultured into confocal dishes and performed different cotransfections of PBIND vector, PACT-MyoD control vector, pG5luc vector, pBIND-ALDH2, pACT-HSF1, and pACT-HSPA1A. RLU (relative light unit) was measured by the Modulus determinator (Turner Biosystems, USA).
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