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Thermal tail-flick test

BT Biao Tang
DL Dan Liu
LC Lingyu Chen
YL Yu Liu
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The thermal tail-flick test was used to assess the nociceptive reflex response to thermal stimulus in treated animals. When the pain threshold of mice is exceeded, they use an effective tail flick escape [20]. The thermal tail-flick test of acute pain was performed on the 11th day after pharmaceutical injection. Each experimental mouse was placed in a fixator with the tail exposed. When it was stable, 1/3rd of the tail was placed in hot water at 52 °C. The tail-flick latency (from the tail entering the water to exiting) was measured with a stopwatch (accuracy: 0.01 s) for 4 consecutive times each with an interval of 1 min [21]. Each tail was measured in triplicate, and the average value was calculated.

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