2.7. Measurement of intestinal transit time and length

HL Hyun‐Joo Lee
EC Eun Joo Choi
SP Sihoon Park
JL Jae‐Joon Lee
ask Ask a question
Favorite

The gastrointestinal transit time of feed consumed by the rats was measured using the method of Hinton et al. (1969), with minor modification. The animals were fasted for 12 hr on the sixth day after administration of loperamide. Rats were then fed a diet mixed with 1 g of 10% Coomassie brilliant blue dye, and the total time taken to defecate the blue‐colored fecal pellets was determined to be the gastrointestinal transit time. Intestinal length was measured by resection (excluding the appendix).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A