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siRNA treatment

MD Madison L. Doolittle
GC Gina M. Calabrese
LM Larry D. Mesner
DG Dana A. Godfrey
RM Robert D. Maynard
CA Cheryl L. Ackert-Bicknell
CF Charles R. Farber
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MC3T3-E1 cells were plated at 10,000 cells/cm2 in 12-well plates and allowed to adhere overnight. The following day the cells were transfected with 5nM scrambled control or Zbtb40 Silencer Select siRNA (ThermoFisher) delivered with Lipofectamine RNAiMAX (ThermoFisher). Knockdown efficiency was determined by measuring RNA (24 hours) or protein (72 hours) expression levels post-transfection. Remaining cells were then induced with osteogenic medium at 100% confluency (72 hours post-transfection) and differentiated until the indicated time point for phenotype analysis.

Copyright and License information:  ©2020 Doolittle et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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