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Culture medium for EpiLCs induction33 was DMEM/F12 + Neurobasal containing B27 and N2 cell supplements, 20 ng/ml recombinant human activin A (R&D Systems), 12 ng/ml bFGF (Wako), 100 U/ml penicillin, 100 μg/ml streptomycin, and 1% KO serum replacement (GIBCO).
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