Nude mouse xenograft tumor tissue sections were prepared and subjected to H&E and Ki-67 IHC staining as described previously 19, 24. Mouse lung normal and tumor tissues resulting from exposure to BaP alone or arsenic plus BaP in our recent study 14 were sectioned and used for MCL-1 IF staining following our published protocols 19, 20. The IHC and IF staining images were taken using Nikon NIS-Elements software.
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