Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Caspase 3 activity was assessed using a Colorimetric Caspase 3 Enzyme Assay Kit (Abcam). Briefly, RF-treated HUVECs were resuspended in lysis buffer and incubated on ice for 30 min. After centrifugation at 14,000 × g for 10 min at 4 °C, protein extracts were collected and quantified using a DC Protein Array Kit (Bio-Rad, Hercules, CA, USA). Protein extracts (500 µg) were incubated with reaction buffer containing 2 mM Ac‐DEVD‐AFC and 10 mM DTT at 37 °C for 30 min. Caspase activity was determined by measuring the absorbance at 405 nm.
Copyright and License information: The Author(s) ©2020
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
