Colon cancer cells were incubated in medium without FBS for 12 h and fixed with 75% ethanol for 6 h at 4 °C. Fixed cells were incubated with propidium iodide (50 μg/mL) containing RNase A (0.1 mg/mL) for 30 min at room temperature. The stained cells were then analyzed by flow cytometry (BD Biosciences).
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