Cell viability assays were performed using a Cell Counting Kit (CCK) (FC101-02, TransGen Biotech, Beijing, China) according to the manufacturer’s protocol. Briefly, 5×103 cells per well were placed into 96-well plates, 10 µl CCK solution was added at the indicated time, and the plates were incubated for 2 hours in a cell culture incubator. Absorbance was measured at 450 nm using a microplate reader.
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