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2.4. Annexin V/PI staining and flow cytometry analysis

CH Cheng Hu
HX Hongyan Xia
SB Shanshan Bai
JZ Jianlei Zhao
HE Holly Edwards
XL Xinyu Li
YY Yanrong Yang
JL Jing Lyu
GW Guan Wang
YZ Yang Zhan
YD Yan Dong
YG Yubin Ge
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Prostate cancer cells were treated with the indicated drugs. Drug‐induced apoptosis was determined using the Annexin V‐Fluorescein Isothiocyanate (FITC)/Propidium Iodide (PI) Apoptosis Kit (Beckman Coulter) by following the manufacture's protocol. The experiments were repeated at least three times. Mean percentage of AnnexinV+/PI‐ (early apoptotic) and Annexin V+/PI+ (late apoptotic and/or dead) ± standard errors from triplicates of one representative experiment is shown.

Copyright and License information:  ©2020 The Authors. published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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