Euglobulin clot-lysis time.

A total of 50 μL of citrated mouse plasma was resuspended in 900 μL 0.017% acetic acid, placed on ice for 20 minutes, and then centrifuged for 20 minutes at 2000 g at 4°C. After careful removal of the supernatant fraction, each pellet (euglobulin fraction) was resuspended in 55 μL sodium borate/NaCl (pH 9.0) and transferred to a single well on a flat‑bottom 96-well microtiter assay plate; 50 μL of 25 mM CaCl₂ was added to each well, and then the absorbance was recorded at 405 nm every 10 minutes, with 3-second shakes before each reading, at room temperature for 16 hours. Clot-lysis time was calculated as the time to achieve 50% of clot lysis (half-lysis time) (80).